polyclonal rabbit anti human egfr igg  (Santa Cruz Biotechnology)

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

Article Snippet: Following transfer, EGFR, Nectin-4 and TROP-2 were detected using polyclonal rabbit anti-human EGFR IgG (#sc-03-G, Santa Cruz Biotechnology, Dallas, TX, USA), polyclonal rabbit anti-human Nectin-4 IgG (#17402S, Cell Signaling Technologies, Danvers, MA, USA), and monoclonal rabbit anti-human TROP-2 IgG (#90540, Cell Signaling Technologies).

Techniques: Western Blot, Expressing, Negative Control, Binding Assay, Flow Cytometry, Control

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Graphical abstract illustrating the future clinical application of PIT using WB692-CB2 conjugates for the treatment of NMIBC. Following intravesical application of the antibody–dye conjugates into the bladder, they can be activated by cystoscopic red light for the selective eradication of BC cells expressing EGFR, Nectin-4 and/or TROP-2. For personalized PIT, a pre-therapeutic determination of target antigen expression in biopsy samples may guide the selection of an optimal conjugate combination. Created in BioRender. Wolf, I (2025) https://BioRender.com/sx866ld .

Article Snippet: Following transfer, EGFR, Nectin-4 and TROP-2 were detected using polyclonal rabbit anti-human EGFR IgG (#sc-03-G, Santa Cruz Biotechnology, Dallas, TX, USA), polyclonal rabbit anti-human Nectin-4 IgG (#17402S, Cell Signaling Technologies, Danvers, MA, USA), and monoclonal rabbit anti-human TROP-2 IgG (#90540, Cell Signaling Technologies).

Techniques: Expressing, Selection

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Sorcin regulates alveolarization and airway tissue remodeling during lung morphogenesis

doi: 10.1007/s00018-025-05870-y

Figure Lengend Snippet: Altered protein expression and EGFR signaling in Sorcin-deficient lung development A ) Confocal microscopy of 3-week-old lung tissue from WT and Sri⁻ / ⁻ mice. Sri⁻ / ⁻ lung tissue shows reduced SP-B (red) expression compared to WT. Scale bars, 20 μm B ) Western blot analysis of lung lysates from 3-week-old WT and Sri⁻ / ⁻ mice. Sri⁻ / ⁻ mice exhibit decreased EGFR, PANRAS and RAB5C protein levels compared to WT. Densitometry quantification is shown (mean ± SEM). ** p < 0.01 (Student’s t-test), *** p < 0.001 (determined by Student’s t-test) C-E ) Confocal microscopy of 3-week-old lung sections: WT mice show epithelial localization of EGFR (green) (C), RAB5C (green) (D) and PANRAS (red) protein (E). In Sri⁻ / ⁻ mice, these proteins are significantly reduced. Scale bars, 20 μm

Article Snippet: Then, sections were blocked in 5% goat serum for 1 h and incubated overnight at 4 °C with primary antibody rabbit polyclonal EPCAM/CD326 (1:100 in 1%BSA-PBS solution) (Proteintech, #21050-1-AP), rabbit polyclonal SFTPB (1:100 in 1%BSA-PBS solution) (Thermo Fisher Scientific, # PA542000 ), rabbit polyclonal E-cadherin (1:100 in 1%BSA-PBS solution) (Proteintech, #20874-1-AP), mouse monoclonal E-cadherin (1:100 in 1%BSA-PBS solution) (BD Bioscience, #610181), mouse monoclonal α-Smooth Muscle Actin (1:100 in 1%BSA-PBS solution) (Sigma-Aldrich, #A5228), rabbit polyclonal EGFR (1:100 in 1%BSA-PBS solution) (Proteintech, #30847-1-AP) rabbit polyclonal RAB5C (1:100 in 1%BSA-PBS solution) (Thermo Fisher Scientific, #PA5101828), mouse monoclonal PANRAS (Ab3) (1:100 in 1%BSA-PBS solution) (Sigma-Aldrich, #OP40) followed by incubation with Alexa fluor 488 (rabbit)-conjugated secondary antibodies (1:500 in 1%BSA-PBS solution) (Thermo Fisher Scientific).

Techniques: Expressing, Confocal Microscopy, Western Blot